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|
S/N
|
Primer
|
Annealing temperature (°C)
|
Primer sequences
|
Amplification efficiency
|
|---|
|
1
|
UBC-812
|
47 °C
|
(GA)8A
|
Excellent
|
|
2
|
UBC-817
|
48 °C
|
(CA)8A
|
Poor
|
|
3
|
UBC-818
|
47 °C
|
(CA)8G
|
No band
|
|
4
|
UBC-826
|
47 °C
|
(AC)8C
|
No band
|
|
5
|
UBC-834
|
47 °C
|
(CA)8AG
|
Excellent
|
|
6
|
UBC-835
|
48 °C
|
(AG)8YC
|
Very good
|
|
7
|
UBC-839
|
49 °C
|
(TA)8RG
|
Poor
|
|
8
|
UBC-841
|
48 °C
|
(GA)8YC
|
Excellent
|
|
9
|
UBC-844
|
48 °C
|
(AG)8YT
|
Excellent
|
|
10
|
UBC-848
|
48 °C
|
(CA)8RG
|
No band
|
|
11
|
UBC-851
|
49 °C
|
(GT)8YG
|
Poor
|
|
12
|
UBC-852
|
48 °C
|
(AC)8T
|
Poor
|
|
13
|
UBC-854
|
49 °C
|
(TC)8RG
|
Excellent
|
|
14
|
UBC-857
|
49 °C
|
(AC)8AYG
|
Excellent
|
|
15
|
UBC-860
|
52 °C
|
(TG)8RA
|
Poor
|
|
16
|
UBC-864
|
48 °C
|
(CA)8RT
|
Very poor
|
|
17
|
UBC-865
|
47 °C
|
(CCG)6
|
Poor
|
|
18
|
UBC-873
|
45 °C
|
(GACA)4
|
No band
|
|
19
|
UBC-878
|
48 °C
|
(GGAT)4
|
Poor
|
|
20
|
UBC-879
|
48 °C
|
(CTTCA)3
|
Poor
|
|
21
|
UBC-866
|
55 °C
|
(CTC)6
|
Good
|
|
22
|
UBC-868
|
55 °C
|
(GAA)6
|
Excellent
|
|
23
|
UBC-880
|
48 °C
|
(GGAGA)3
|
Excellent
|
|
24
|
UBC-881
|
49 °C
|
(GGTG)3
|
No band
|
|
25
|
UBC-888
|
47 °C
|
BDB(CA)7
|
Poor
|
- Source: Primer kit 900 (UBC 900); Single-letter abbreviations for mixed base positions: R = (A, G); Y = (C, T)
