Table 2 Primer sequences, PCR conditions and PCR product sizes of ZFX/ZFY, HMG and amelogenin (AMEL) loci
From: Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro
| Gene | Primer sequence | T (°C) a | Product size (bp) | |
|---|---|---|---|---|
| Male | Female | |||
| ZFX/Yb | ||||
| Forward | 5΄-ATAATCACATGGAGAGCCACAAGCT-3΄ | 58 | 445 | 445 |
| Reverse | 5΄-GCACTTCTTTGGTATCTGAGAAAGT-3΄ | 58 | ||
| HMG box of SRY genec | ||||
| Forward | 5΄-TGAACGCTTTCATTGTGTGGTC-3΄ | 58 | 162 | - |
| Reverse | 5΄-GCCAGTAGTCTCTGTGCCTCCT-3΄ | 58 | ||
| Beta-actinc | ||||
| Forward | 5΄-CCTTCAACACCCCTGCCATG-3΄ | 58 | 298 | 298 |
| Reverse | 5΄-TGCTCGAAGTCCAGGGCC-3΄ | 57 | ||
| Amelogenin (AMEL)d | ||||
| Forward | 5΄-TTCTCACCAGTACCCTTCCTA-3΄ | 55 | 395 | 458 |
| Reverse | 5΄-TCAGAGGCAGGTCAGGAAGCA-3΄ | 55 | ||